Von Willebrand Disease (VWD)
Part 2: NHLBI Diagnosis Guidelines

Questions?

June 2011

Please let us know your comments and suggestions regarding to our new VWF testing algorithm. Please also check Hot Topic lectures by Drs. William Nichols and Rajiv Pruthi regarding other aspects of the VWD laboratory testing. This concludes my talk, and I thank you for your attention.

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Jump to section:

• Introduction
• Objectives
• History of VWF Ristocetin Cofactor Activity (VWF:RCo) Assay
• Problems with VWF:RCo Assay
• Summary of External Quality Assessment of VWF:RCo
• von Willebrand Disease(VWD) Diagnostic Error Rates Due to Erroneous VWF:RCo Results⁵
• VWF:RCo/Ag Ratio is Insensitive for Aquired von Willebrand Abnormalities (AVWA)
• VWF:RCo is Inadequate for Detecting AVW Abnormality
• The New Automated VWF Activity by Latex-Immunoturbidity Assay (VWF:Lx)
• VWF Activity Assay by an A1 Domain Conformation-Sensitive Antibody⁶
• Performance Characteristics of VWF:Lx
• VWF:Lx and VWF:RCo Method Comparison
• VWF:Lx and VWF:RCo Bias Analysis
• Sample Categorization
• Comparison of VWF:Lx/Ag and VWF:RCo/Ag Ratios
• VWF:Lx and VWF:RCo Errors
• Severe Type 1 vs 2M⁷
• VWF:Lx and RCo Errors
• VWF:Lx/Ag is Sensitive to AVWA with Subtle Loss of Highest HMWM
• ROC Analysis of VWF:Lx/Ag and VWF:RCo/Ag for Detecting AVWA with Loss of HMWMs
• VWF:Lx/Ag vs VWF:RCo/Ag Ratios
• VWF Laboratory Testing Strategy
• Sensitivity and Specificity of the Screening Panel
• von Willebrand Disease Profile
• von Willebrand Disease Profile
• von Willebrand Disease Profile
• von Willebrand Disease Profile
• Limitations of VWF:Lx
• Summary
• References
• Questions?