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Von Willebrand Disease (VWD)
Part 3: VWF Activity Assay

A New von Willebrand Factor Activity Assay and Testing Algorithm



VWF:Lx and VWF:RCo Errors

Slide 16

June 2011

Now let us examine the outliers in detail. 2 of the 3 samples of the normal group and type 2A or 2B group were lipemic. As we know that lipemia could potentially interfere with the optical property of a turbidimetric assay. However, the discrepancy of the remaining 1 sample from the normal and the 1 sample from the type 2A or 2B group can not be clinically verified since they were both Mayo Medical Laboratories samples. This underscores a potential limitation of this assay that it is still uncertain if the new assay will detect all type 2 von Willebrand disease variants, or conversely, if the new assay will pick up new type 2 von Willebrand variants that would have been missed by the VWF ristocetin cofactor activity assay. As for the group of severe types 1 or 2M, we classify the 8 samples by using a highly sensitive flow cytometric method.

VWF:Lx and VWF:RCo Errors

 


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