Von Willebrand Disease (VWD)
Part 2: NHLBI Diagnosis Guidelines

VWF Multimer Analysis: Intermediate Resolution³

May 2011

This slide provides an example of qualitative imaging analysis of plasma VWF multimers, using "intermediate resolution" agarose gel electrophoresis with immunostain infra-red imaging showing high molecular weight multimers near the top and lower molecular weight multimers (LMWM) near the bottom of electrophoresis sample lanes.

Typical results are shown for: a) Normal plasma (in the right-hand lane); b) Type 2A (or 2B) VWD with absence of HMWM, in the next lane to the left; c) Type 3 (severe) VWD with no VWF multimers or protein, in the center lane; d) Type 1 VWD where all multimers are present with normal distribution, but VWF protein (antigen) is low; and f) in the far left lane, an unusual type 2M VWD plasma with no loss of high molecular weight multimers but with ultra-large VWF multimers as well as “smeariness” of high molecular weight multimers, reflecting a mutation substituting cysteine (C) in place of arginine (R) at amino acid 1374 in full length VWF (R1374C).

< Previous Slide

VWF Multimer Analysis: Intermediate Resolution3

Next Slide >

 

Jump to section:

• Introduction
• Objectives—Part 2
• VWD NHLBI Guidelines (2008)¹
• VWD Classification — ISTH^1,2
• VWD NHLBI Guidelines (2008) Initial Patient Evaluation — Laboratory¹
• VWD NHLBI Guidelines 2008 Initial Patient Evaluation — Laboratory¹
• VWD — Initial Laboratory Evaluation
• VWD NHLBI Guidelines (2008) Subsequent Patient Evaluation—Laboratory¹
• Additional VWD Testing — Laboratory¹
• VWF Multimer Analysis: Intermediate Resolution³
• VWF Multimer Analysis: Recommendations⁴
• VWF Multimer Analysis: Recommendations⁴
• Expected Laboratory Values in VWD¹
• Typical Patterns of Laboratory Test Results for VWD Diagnosis and Classification¹
• VWD NHLBI Guidelines (2008) "Low VWF" versus "VWD Type 1"
• VWD Diagnosis (Especially Type 1)
• VWD NHLBI Guidelines ABO Blood Group & VWF/VWD^1,5
• VWD NHLBI Guidelines ABO Blood Group and VWF/VWD: Soft Recommendations
• VWD NHLBI Guidelines (2008): Collection and Handling of Samples for Laboratory Testing¹
• VWD NHLBI Guidelines (2008): Collection and Handling of Samples for Laboratory Testing¹
• VWD NHLBI Guidelines (2008): Collection and Handling of Samples for Laboratory Testing¹
• VWD NHLBI Guidelines: Collection and Handling of Samples for Laboratory Testing^1,4
• VWD NHLBI Guidelines (2008) Summary of Laboratory Recommendations
• VWD NHLBI Guidelines (2008) Summary of Laboratory Recommendations
• References
• VWD Profile
• Questions?