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Von Willebrand Disease (VWD)
Part 2: NHLBI Diagnosis Guidelines

Laboratory Recommendations



VWF Multimer Analysis: Intermediate Resolution3

Slide 10

May 2011

This slide provides an example of qualitative imaging analysis of plasma VWF multimers, using "intermediate resolution" agarose gel electrophoresis with immunostain infra-red imaging showing high molecular weight multimers near the top and lower molecular weight multimers (LMWM) near the bottom of electrophoresis sample lanes.

Typical results are shown for: a) Normal plasma (in the right-hand lane); b) Type 2A (or 2B) VWD with absence of HMWM, in the next lane to the left; c) Type 3 (severe) VWD with no VWF multimers or protein, in the center lane; d) Type 1 VWD where all multimers are present with normal distribution, but VWF protein (antigen) is low; and f) in the far left lane, an unusual type 2M VWD plasma with no loss of high molecular weight multimers but with ultra-large VWF multimers as well as “smeariness” of high molecular weight multimers, reflecting a mutation substituting cysteine (C) in place of arginine (R) at amino acid 1374 in full length VWF (R1374C).

VWF Multimer Analysis: Intermediate Resolution3

 


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