Circulating Tumor Cells and the CellSearch Assay

Labeling and Identification of Cells

April 2011

This is a graphical representation of the staining pattern. As you can see, the circulating tumor cells are identified by a positive EpCam, positive cytokeratin, and a positive DAPI pattern. In turn, a leukocyte is positive for CD45 and DAPI, but negative for cytokeratin staining. These staining patterns are then used by a trained technologist to interpret images that are presented in a gallery on the Celltracks analyzer.

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Labeling and Identification of Cells

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Jump to section:

• Introduction
• Circulating Tumor Cell (CTC)
• The Metastatic Process
• Needle in a Haystack...
• Circulating Tumor Cells
• The CellSearch System (Veridex LLC)
• Automated Sample Preparation
• Labeling and Identification of CTCs
• Labeling and Identification of Cells
• Cell Analysis
• Mayo Medical Laboratories FDA-Cleared Assays
• Metastatic Breast Cancer (MBC)
• CellSearch Use in MBC Clinical Trial⁶
• Progression-Free Survival in MBC
• Overall Survival in MBC
• Metastatic Colorectal Cancer (MCRC)
• CellSearch Use in MCRC Clinical Trial⁸
• Progression-Free Survival in MCRC
• Overall Survival in MCRC
• Metastatic Prostate Cancer (MPC)
• CellSearch Use in MPC Clinical Trial⁸
• Progression-Free Survival in MPC
• Overall Survival in MPC
• The Future of CTC
• References
• Questions?