Circulating Tumor Cells and the CellSearch Assay
Labeling and Identification of CTCs
April 2011
Besides using the EpCam antibodies for immunomagnetic labeling and capturing of the epithelial cells, the CellSearch System also uses 3 stains to help distinguish true CTCs from contaminating leukocytes. Circulating tumor cells are identified by using antibodies labeled with phycoerythrin (PE) to cytokeratins 8, 18, and 19. Leukocytes are marked using allophycocyanin (APC)-labeled antibodies to CD45. Additionally, DAPI stains the nuclei of cells and helps distinguish viable cells from nonspecific debris.
Labeling and Identification of CTCs |
Jump to section:
- Introduction
- Circulating Tumor Cell (CTC)
- The Metastatic Process
- Needle in a Haystack...
- Circulating Tumor Cells
- The CellSearch System (Veridex LLC)
- Automated Sample Preparation
- Labeling and Identification of CTCs
- Labeling and Identification of Cells
- Cell Analysis
- Mayo Medical Laboratories FDA-Cleared Assays
- Metastatic Breast Cancer (MBC)
- CellSearch Use in MBC Clinical Trial6
- Progression-Free Survival in MBC
- Overall Survival in MBC
- Metastatic Colorectal Cancer (MCRC)
- CellSearch Use in MCRC Clinical Trial8
- Progression-Free Survival in MCRC
- Overall Survival in MCRC
- Metastatic Prostate Cancer (MPC)
- CellSearch Use in MPC Clinical Trial8
- Progression-Free Survival in MPC
- Overall Survival in MPC
- The Future of CTC
- References
- Questions?
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