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Circulating Tumor Cells and the CellSearch Assay



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Labeling and Identification of CTCs

Slide 8

April 2011

Besides using the EpCam antibodies for immunomagnetic labeling and capturing of the epithelial cells, the CellSearch System also uses 3 stains to help distinguish true CTCs from contaminating leukocytes. Circulating tumor cells are identified by using antibodies labeled with phycoerythrin (PE) to cytokeratins 8, 18, and 19. Leukocytes are marked using allophycocyanin (APC)-labeled antibodies to CD45. Additionally, DAPI stains the nuclei of cells and helps distinguish viable cells from nonspecific debris.

Labeling and Identification of CTCs

 


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