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Circulating Tumor Cells and the CellSearch Assay



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Automated Sample Preparation

Slide 7

April 2011

This slide shows in detail the sample preparation that takes place in the Autoprep. 7.5 mL of blood is drawn into Cellsave tubes that contain a preservative that allows for sample to be stored at room temperature for 96 hours. Seven point five mL of blood is removed and placed in a processing tube and centrifuged at 800g for 10 minutes. That tube is then placed on the Autoprep for processing. The plasma is aspirated and buffer containing ferrofluid compounds that are bound to EpCam antibodies are added.

Magnets are then engaged which draws the ferrofluid particles to the side of the tube, leaving any unlabeled cells to be aspirated. The magnets are then removed and target cells are resuspended in buffer that contains permeabilizing and staining reagents. This epithelial-enriched sample is then placed in a cell presentation device called a Magnest that attracts the magnetically labeled epithelial cells to the surface of the cartridge.

Automated Sample Preparation

 


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