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Fungal Molecular Diagnostics


Histoplasma capsulatum/Blastomyces dermatitidis Real-Time PCR

Slide 30

February 2011

The last PCR assay that I will discuss briefly is a new one that will be introduced this year. That assay allows for the detection and identification of Histoplasma capsulatum and Blastomyces dermatitidis in a single tube either from culture isolates or directly from patient respiratory or tissue specimens. We recently submitted a manuscript describing the assay for peer-review evaluation. The use of distinctly labeled fluorophores on the FRET probes allows for the detection and differentiation of Histoplasma capsulatum and Blastomyces dermatitidis from a single tube using different detection wavelengths. We are very excited about this assay since we will be able to rapidly detect and distinguish between these 2 dimorphic pathogens that have overlapping areas of endemicity and that often can have overlapping clinical pictures.

H. capsulatum/B. dermatitidis PCR

 


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