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Fungal Molecular Diagnostics


Melt-curve Analysis of Coccidioides spp Grown in Culture

Slide 27

February 2011

Similar to the Pneumocystis assay, the Coccidioides PCR assay utilizes sequence-specific FRET probes and melt-curve analysis for detection. A melt peak occurs at approximately 60ºC with no cross-reactions occurring with any other respiratory pathogens including bacteria, viruses, and other fungi.

Melt-curve Analysis of Coccidioides spp

 


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