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Fungal Molecular Diagnostics

Concordance of the Pneumocystis LightCycler PCR Assay and Calcofluor White Stain for BAL Fluid

Slide 25

February 2011

Verification of the sensitivity and specificity of the Pneumocystis PCR assay against a calcofluor white stain method that has been used for many years in our laboratory, indicated that the PCR assay had a 22% increase in sensitivity relative to the stain, with the PCR assay detecting more positives from BAL fluid than did the stain.

LightCycler Assay & Calcofluor White Stain


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