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Fungal Molecular Diagnostics

Real-time PCR Workflow

Slide 22

February 2011

The workflow for all of our real-time PCR assays is the same and is depicted in this slide. We begin with the patient specimen, which undergoes a processing step to lyse and inactivate the organism. Next the specimen is extracted to isolate the fungal nucleic acids and the nucleic acid is amplified using real-time PCR. Sequence-specific detection is achieved using fluorescence resonance energy transfer (or FRET) probes. The entire process requires about 4 hours from start to finish and, therefore, the result can be available the same day that the specimen is submitted.

Real-time PCR Workflow


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