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Fungal Molecular Diagnostics


Fungal PCR Assays at Mayo Clinic

Slide 21

February 2011

Now that we have discussed the utility of nucleic acid hybridization probes, DNA sequencing, and PNA FISH probes for assisting in identification of fungi growing in culture, let’s switch gears and discuss molecular methods available for the direct detection of fungi in clinical specimens without the need for culture. Currently this is a very, very short list of methods consisting of a handful of laboratory-developed PCR assays that target specific fungi. In our laboratory, we have 2 of these real-time PCR assays that have been used routinely for several years. The first assay provides for the direct detection of Pneumocystis jiroveci from respiratory specimens such as bronchoalveolar lavage fluid and respiratory tissues. The second assay allows for the direct detection of Coccidioides immitis and Coccidioides posadasii from respiratory specimens, body fluids, and tissues. Within the next few months, we will begin offering a third real-time PCR assay that provides a single tube assay for detection and differentiation of Histoplasma capsulatum and Blastomyces dermatitidis from respiratory specimens, body fluids, and tissue.

Fungal PCR Assays at Mayo Clinic

 


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