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Fungal Molecular Diagnostics

PNA FISH for Candida spp—Direct Detection from Blood

Slide 18

February 2011

After the Gram stain and the hybridization process is complete, Candida albicans and Candida parapsilosis are identified microscopically as bright green fluorescing cells, Candida tropicalis fluoresces bright yellow, and Candida glabrata and Candida krusei fluoresce bright red. Other yeast do not fluoresce. In addition to providing identification of the 5 most common species of Candida, the Traffic Light probes also provide an indication about the potential utility of fluconazole in these patients since Candida albicans and Candida parapsilosis are generally susceptible to fluconazole, Candida glabrata can be resistant to fluconazole and Candida krusei is intrinsically resistant to fluconazole. Therefore, a green fluorescent signal can serve as a "go" indication for the clinician to use fluconazole, while a red fluorescent signal serves as a "stop" warning sign because fluconazole may not be a good choice for this patient. Finally, the yellow signal produced by Candida tropicalis indicates that caution should be used since fluconazole susceptibility is variable for this organism.

PNA FISH Direct Detection from Blood


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