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Fungal Molecular Diagnostics


Current Applications of D2 Fungal Sequencing at the Mayo Clinic

Slide 15

February 2011

Not every yeast or mold identification requires DNA sequencing. Many identifications are made quite easily and cost-effectively using traditional methods. Here at Mayo, we selectively utilize D2 DNA sequencing as a tool when traditional methods aren't providing a clear answer or when traditional methods require inordinate amounts of time. For example, it has been very helpful for some yeast that are difficult to identify using phenotypic methods such as the API20C assimilation test. Further, we have had great success using it to rapidly rule-out pathogens when fungi are persistently nonsporulating, as often occurs in the summer months. D2 sequencing has been very helpful for several cases of difficult to identify molds. And finally, D2 sequencing is extremely useful to confirm questionable nucleic acid hybridization probe results in instances where one may be considering a false-positive probe due to a cross-reacting organism. Where necessary, dermatophyte identification can be achieved an average of 9 days faster using sequencing as opposed to phenotypic methods.

Current Applications of D2 Fungal Sequencing

 


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