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Fungal Molecular Diagnostics

Nucleic Acid Hybridization Probes

Slide 8

February 2011

This slide provides an overview of how the nucleic acid hybridization probes function. The laboratorian obtains an RNA template from the organism to be identified by lysing a small amount of the organism after it is picked from a culture plate using an inoculating loop. A DNA-hybridization probe labeled with a chemiluminescent moiety is added to the solution containing the RNA template. If the hybridization probe sequence is complementary to the RNA target, it will bind tightly and specifically. Then, any unbound probe remaining in the solution is hydrolyzed to remove the potential for nonspecific signal and the DNA-RNA hybrid is detected by a chemiluminescence reaction.

Nucleic Acid Hybridization Probes


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