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Fungal Molecular Diagnostics


Traditional Methods of Fungal Diagnostics

Slide 3

February 2011

Before we begin discussing the use of molecular diagnostics, let us recall that until very recently, the methods used by mycologists for the identification of fungi in the laboratory have been largely unchanged for decades. Once received in the laboratory, a patient specimen will typically be plated onto several different types of media and these media will be incubated at a constant temperature for up to 4 weeks. Laboratory staff will periodically examine the plates for growth and when growth is noted, the staff will try to identify the fungus using both macroscopic and microscopic morphologies. Often, subculture of the fungus to other types of media, followed by additional incubation time may be required in order to induce sporulation or the development of characteristic structures that can be used in making a visual identification. If identification cannot be made based on morphology alone, there are a few biochemical tests that can sometimes be helpful in identification, most often for yeast.

Traditional Methods of Fungal Diagnostics

 


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