Specimen Minimum Volume

25-OH Vitamin D: Minimum Volume Requirements

January 2011

An example of this is our vitamin D test. 25-Hydroxyvitamin D is the preferred test for the evaluation of possible vitamin D deficiency or excess. For many years, we used high performance liquid chromatography (abbreviated HPLC) to do the test, and that method had a very high volume requirement—originally, 3 mL of serum was the minimum amount needed to perform the test. That volume requirement pushed our scientists to develop an assay that did not require so much sample. Subsequently, we replaced the HPLC vitamin D assay with a mass spectrometry assay that is analytically superior AND can be done on as little as 0.25 mL.

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25-OH Vitamin D: Minimum Requirements

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• Introduction
• Iatrogenic Anemia
• Adverse Consequences of Anemia
• Specimen Volumes Have Decreased Over Time
• Specimen Volume Reduction Initiatives at Mayo
• Specimen Minimum Volume (SMV) Definitions
• Automated Assay SMV
• Manual Assay SMV
• Locating Minimum Volume on the Test Catalog
• Specimen Minimum Volume
• Comparison to 5 Commercial Labs
• Specimen Volume Review
• 25-OH Vitamin D: Minimum Volume Requirements
• Specimen Required or Minimum Volume
• Specimen Required Field
• Minimum Volume Field
• Submitted Volume
• Questions?