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25-OH Vitamin D: Minimum Volume Requirements

Slide 13

January 2011

An example of this is our vitamin D test. 25-Hydroxyvitamin D is the preferred test for the evaluation of possible vitamin D deficiency or excess. For many years, we used high performance liquid chromatography (abbreviated HPLC) to do the test, and that method had a very high volume requirement—originally, 3 mL of serum was the minimum amount needed to perform the test. That volume requirement pushed our scientists to develop an assay that did not require so much sample. Subsequently, we replaced the HPLC vitamin D assay with a mass spectrometry assay that is analytically superior AND can be done on as little as 0.25 mL.

25-OH Vitamin D: Minimum Requirements


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