Laboratory Diagnosis of Shiga-Toxin Producing Escherichia coli

Introduction

December 2010

Welcome to Mayo Medical Laboratories' Hot Topics. These presentations provide short discussions of current topics and may be helpful to you in your practice.

Our presenter for this program is Dr. Thomas Grys, Assistant Laboratory Director, Mayo Medical Laboratories New England, Department of Laboratory Medicine and Pathology at Mayo Clinic. Dr. Grys will discuss Shiga toxin-producing Escherichia coli and methods to detect these strains. He will also review the recent Centers for Disease Control and Prevention (CDC) guidelines on this topic.

Introduction

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Jump to section:

• Introduction
• Escherichia coli
• Diarrheagenic Escherichia coli
• Terms
• STEC Infections
• Clinical Presentation
• STEC Severity and Prevalence
• Culture
• Antigen Detection
• Molecular Methods
• PCR for STEC
• CDC Recommendations¹
• Mayo Real-Time PCR Assay
• Performance of the Real-Time PCR Assay⁷
• Method Comparison
• Clinical Experience with the Real-Time PCR Assay⁸
• Clinical Experience with the Real-Time PCR Assay, cont.⁸
• Assay Reporting
• Summary
• Acknowledgements
• References
• Questions?