Paroxysmal Nocturnal Hemoglobinuria
Normal
December 2010
This slide shows flow cytometry dot-plots of a normal individual without a PNH clone. Without going into details regarding gating strategies, I just want to emphasize that the different cell populations are much more cleanly separated and that we collect many more events, increasing our sensitivity to 0.01% for RBCs and granulocytes and 0.05% for monocytes. Important new element is also that we are able to separate type II RBCs which although have partial loss of GPI-linked proteins on the cell surface, are not susceptible to hemolysis.
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Jump to section:
- Introduction
- Paroxysmal Nocturnal Hemoglobinuria
- Prevalence
- Pathogenesis
- Paroxysmal Nocturnal Hemoglobinuria
- Progression
- New Developments
- New Developments, cont.
- Role of Laboratory Testing
- International PNH Group Recommendations: Who to Test?
- Diagnosis
- International PNH Group Recommendation: How to Test?
- Normal
- New WBC PNH
- Sensitivity and Reference Interval
- Interpretation and Reporting
- PNH—Summary
- Questions?
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