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Paroxysmal Nocturnal Hemoglobinuria



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Slide 13

December 2010

This slide shows flow cytometry dot-plots of a normal individual without a PNH clone. Without going into details regarding gating strategies, I just want to emphasize that the different cell populations are much more cleanly separated and that we collect many more events, increasing our sensitivity to 0.01% for RBCs and granulocytes and 0.05% for monocytes. Important new element is also that we are able to separate type II RBCs which although have partial loss of GPI-linked proteins on the cell surface, are not susceptible to hemolysis.

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