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Paroxysmal Nocturnal Hemoglobinuria


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International PNH Group Recommendation: How to Test?

Slide 12

December 2010

Besides recommendations who to test, International PNH group came up with recommendations on how to perform flow cytometry testing. The main goal was to try to standardize testing amongst different laboratories. Some of the highlights include use of at least 2 different antibodies against 2 different surface proteins on 2 different cell populations. Also it is recommended to use antibodies for gating, instead of forward and side scatter, as well as minimum sensitivity of 1%.

With these recommendations in mind, we have decided to update our PNH test.

How to Test?


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