Paroxysmal Nocturnal Hemoglobinuria
Diagnosis
December 2010
How to test for PNH? The Ham test for sensitivity of red blood cells to complement has only a historical importance these days, as it is laborious, and lacks sensitivity and specificity. Since early 1990’s flow cytometry immunophenotyping has been the mainstay of PNH testing. Most tests were designed to look for the loss of CD59 and CD55 on RBC, loss of CD59 and fluorescent aerolysin binding to granulocytes, and loss of CD14 on monocytes. Fluorescent aerolysin or FLARE, is a modified Pseudomonas protein, that binds to GPI link.
Diagnosis |
Jump to section:
- Introduction
- Paroxysmal Nocturnal Hemoglobinuria
- Prevalence
- Pathogenesis
- Paroxysmal Nocturnal Hemoglobinuria
- Progression
- New Developments
- New Developments, cont.
- Role of Laboratory Testing
- International PNH Group Recommendations: Who to Test?
- Diagnosis
- International PNH Group Recommendation: How to Test?
- Normal
- New WBC PNH
- Sensitivity and Reference Interval
- Interpretation and Reporting
- PNH—Summary
- Questions?
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