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Leukocyte Alkaline Phosphatase Stain (LAP)

Optimizing Laboratory Testing for Hematologic
Disorders Series

LAP Procedure—an Inexact Science!

Slide 8

September 2010

The LAP assay is clearly a subjective test based on an inexact science. The procedure involves counting 100 neutrophils including lobed and band forms but excluding other left-shifted granulocytes, eosinophils, and basophils. The reaction is scored from 0 to 4 depending on the number of stained granules and the intensity of the stain. The number of cells is multiplied by the score and added up with a normal range being from 40 to 100.

LAP Procedure


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