Laboratory Diagnosis of Tick-Borne Infections
Part 2
Conclusions, continued
July 2010
Some specific points about PCR is that blood is NOT a preferred specimen for Lyme PCR and it does seem to have a lower sensitivity than other specimens such as synovial fluid and skin tissue for detection of Borrellia burgdorferi DNA. Also, there is a new Ehrlichia species that is closely related to Ehrlichia muris and it has been described so far from humans in Minnesota and Wisconsin and right now we are referring to this as an Ehrlichia muris-like organism and physicians should be aware of the prevalence of tick-borne diseases in their region and then consider those organisms in their differential diagnosis. So with that, I’d like to conclude this presentation.
Conclusions, continued |
Jump to section:
- Introduction
- Presentation Outline
- Tick-Borne Diseases
- Advantages and Limitations of Conventional Tests
- Molecular Diagnostic Methods Potential Advantages
- Molecular Diagnostic Methods Potential Disadvantages
- Treatment and Diagnostic Recommendations
- Case 1
- Diagnostic Workup
- Representative Image Giemsa-stained Peripheral Blood Smear
- Differential Diagnosis
- Which one is Plasmodium falciparum?
- Main Morphologic Features Table
- Classic Maltese Cross
- Extracellular Forms of Babesiosis
- Side-by-Side Comparison
- Further Workup
- Malaria PCR
- Babesia microti PCR
- Advantages/Uses of PCR
- Potential Disadvantages
- Potential Disadvantages
- Case 2
- Lyme Disease: Diagnostic Approach
- Reported Cases of Lyme Disease, 2008
- Serologic Assay: Test of Choice for Diagnosis of Lyme Disease
- Case 2, Further Workup
- Potential Roles for PCR
- Which Specimens Should be Tested by PCR?
- PCR for Detection of Borrelia burgdorferi DNA in Blood
- Case 3
- Live Tick Image
- Tick Image Close Up
- IDSA Guidelines
- IDSA Guidelines, continued
- Testing of Ticks by PCR
- Case 4
- Tick-Borne PCR Panel
- Diagnostic Tests for HME and HGA
- PCR for Diagnosis of Ehrlichiosis/Anaplasmosis
- Ehrlichia and Anaplasma PCR Assay Graph
- Phylogenetic Tree
- Ehrlichia muris-like Organism
- All Patients with EML had Tick Exposure
- Prevention of Tick-Borne Diseases
- Conclusions
- Conclusions, continued
- Questions?
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