Laboratory Diagnosis of Tick-Borne Infections

Prevention of Tick-Borne Diseases

July 2010

So, this brings me then to my last point, which is that prevention of tick-borne diseases is best accomplished by taking some simple precautions, such as wearing protective clothing, including long pants tucked into socks and boots, use of insect repellants, such as those containing DEET, or the use of permethrin-impregnated clothing, and finally, checking for ticks after potential exposures.

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Prevention of Tick-Borne Diseases

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• Introduction
• Presentation Outline
• Tick-Borne Diseases
• Advantages and Limitations of Conventional Tests
• Molecular Diagnostic Methods Potential Advantages
• Molecular Diagnostic Methods Potential Disadvantages
• Treatment and Diagnostic Recommendations
• Case 1
• Diagnostic Workup
• Representative Image Giemsa-stained Peripheral Blood Smear
• Differential Diagnosis
• Which one is Plasmodium falciparum?
• Main Morphologic Features Table
• Classic Maltese Cross
• Extracellular Forms of Babesiosis
• Side-by-Side Comparison
• Further Workup
• Malaria PCR
• Babesia microti PCR
• Advantages/Uses of PCR
• Potential Disadvantages
• Potential Disadvantages
• Case 2
• Lyme Disease: Diagnostic Approach
• Reported Cases of Lyme Disease, 2008
• Serologic Assay: Test of Choice for Diagnosis of Lyme Disease
• Case 2, Further Workup
• Potential Roles for PCR
• Which Specimens Should be Tested by PCR?
• PCR for Detection of Borrelia burgdorferi DNA in Blood
• Case 3
• Live Tick Image
• Tick Image Close Up
• IDSA Guidelines
• IDSA Guidelines, continued
• Testing of Ticks by PCR
• Case 4
• Tick-Borne PCR Panel
• Diagnostic Tests for HME and HGA
• PCR for Diagnosis of Ehrlichiosis/Anaplasmosis
• Ehrlichia and Anaplasma PCR Assay Graph
• Phylogenetic Tree
• Ehrlichia muris-like Organism
• All Patients with EML had Tick Exposure
• Prevention of Tick-Borne Diseases
• Conclusions
• Conclusions, continued
• Questions?