Laboratory Diagnosis of Tick-Borne Infections
Part 2
Ehrlichia and Anaplasma PCR Assay Graph
July 2010
This is a print out of the ehrlichia and anaplasma PCR assay performed at our institution on a Roche Lightcycler instrument. And again, the use of melting curve analysis allows for differentiation of the 3 main organisms that cause human disease. So from left to right, we have Ehrlichia ewingii, Ehrlichia chaffeensis, and Anaplasma phagocytophilum. However, notice this peak here that doesn’t line up with any of the 3 organisms. This is actually the result from this patient. And, it is clearly positive, but it seems to represent a novel species of Ehrlichia.
Ehrlichia and Anaplasma PCR Assay Graph |
Jump to section:
- Introduction
- Presentation Outline
- Tick-Borne Diseases
- Advantages and Limitations of Conventional Tests
- Molecular Diagnostic Methods Potential Advantages
- Molecular Diagnostic Methods Potential Disadvantages
- Treatment and Diagnostic Recommendations
- Case 1
- Diagnostic Workup
- Representative Image Giemsa-stained Peripheral Blood Smear
- Differential Diagnosis
- Which one is Plasmodium falciparum?
- Main Morphologic Features Table
- Classic Maltese Cross
- Extracellular Forms of Babesiosis
- Side-by-Side Comparison
- Further Workup
- Malaria PCR
- Babesia microti PCR
- Advantages/Uses of PCR
- Potential Disadvantages
- Potential Disadvantages
- Case 2
- Lyme Disease: Diagnostic Approach
- Reported Cases of Lyme Disease, 2008
- Serologic Assay: Test of Choice for Diagnosis of Lyme Disease
- Case 2, Further Workup
- Potential Roles for PCR
- Which Specimens Should be Tested by PCR?
- PCR for Detection of Borrelia burgdorferi DNA in Blood
- Case 3
- Live Tick Image
- Tick Image Close Up
- IDSA Guidelines
- IDSA Guidelines, continued
- Testing of Ticks by PCR
- Case 4
- Tick-Borne PCR Panel
- Diagnostic Tests for HME and HGA
- PCR for Diagnosis of Ehrlichiosis/Anaplasmosis
- Ehrlichia and Anaplasma PCR Assay Graph
- Phylogenetic Tree
- Ehrlichia muris-like Organism
- All Patients with EML had Tick Exposure
- Prevention of Tick-Borne Diseases
- Conclusions
- Conclusions, continued
- Questions?
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