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Laboratory Diagnosis of Tick-Borne Infections

Part 2


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Ehrlichia and Anaplasma PCR Assay Graph

Slide 41

July 2010

This is a print out of the ehrlichia and anaplasma PCR assay performed at our institution on a Roche Lightcycler instrument. And again, the use of melting curve analysis allows for differentiation of the 3 main organisms that cause human disease. So from left to right, we have Ehrlichia ewingii, Ehrlichia chaffeensis, and Anaplasma phagocytophilum. However, notice this peak here that doesn’t line up with any of the 3 organisms. This is actually the result from this patient. And, it is clearly positive, but it seems to represent a novel species of Ehrlichia.

Ehrlichia and Anaplasma PCR Assay Graph


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