Laboratory Diagnosis of Tick-Borne Infections
Part 2
Introduction
July 2010
Welcome to Mayo Medical Laboratories' Hot Topics. These presentations provide short discussions of current topics and may be helpful to you in your practice.
Our speaker for this program is Dr. Bobbi Pritt, Assistant Professor of Laboratory Medicine and Pathology and Director of the Clinical Virology and Parasitology Laboratories in the Division of Clinical Microbiology at Mayo Clinic. In Part 2, Dr. Pritt provides an overview of the molecular methods available for detection and diagnosis of tick-borne infections. In Part 1, which is also posted, Dr. Matthew Binnicker discusses the conventional methods available for detection and diagnosis of tick-borne infections.
In this presentation, we’ll discuss the molecular approaches to diagnosis of tick-borne infections, with an emphasis on how PCR can be used to confirm or supplement conventional methods of diagnosis, such as serologic tests. This presentation is a follow-up to the hot topic given by Dr. Binnicker on conventional diagnostic methods of tick-borne disease.
Introduction |
Jump to section:
- Presentation Outline
- Tick-Borne Diseases
- Advantages and Limitations of Conventional Tests
- Molecular Diagnostic Methods Potential Advantages
- Molecular Diagnostic Methods Potential Disadvantages
- Treatment and Diagnostic Recommendations
- Case 1
- Diagnostic Workup
- Representative Image Giemsa-stained Peripheral Blood Smear
- Differential Diagnosis
- Which one is Plasmodium falciparum?
- Main Morphologic Features Table
- Classic Maltese Cross
- Extracellular Forms of Babesiosis
- Side-by-Side Comparison
- Further Workup
- Malaria PCR
- Babesia microti PCR
- Advantages/Uses of PCR
- Potential Disadvantages
- Potential Disadvantages
- Case 2
- Lyme Disease: Diagnostic Approach
- Reported Cases of Lyme Disease, 2008
- Serologic Assay: Test of Choice for Diagnosis of Lyme Disease
- Case 2, Further Workup
- Potential Roles for PCR
- Which Specimens Should be Tested by PCR?
- PCR for Detection of Borrelia burgdorferi DNA in Blood
- Case 3
- Live Tick Image
- Tick Image Close Up
- IDSA Guidelines
- IDSA Guidelines, continued
- Testing of Ticks by PCR
- Case 4
- Tick-Borne PCR Panel
- Diagnostic Tests for HME and HGA
- PCR for Diagnosis of Ehrlichiosis/Anaplasmosis
- Ehrlichia and Anaplasma PCR Assay Graph
- Phylogenetic Tree
- Ehrlichia muris-like Organism
- All Patients with EML had Tick Exposure
- Prevention of Tick-Borne Diseases
- Conclusions
- Conclusions, continued
- Questions?
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