Laboratory Diagnosis of Tick-Borne Infections

Conventional Methods for Diagnosis of Ehrlichiosis and Anaplasmosis

July 2010

This brings us to serology, which is the most commonly used conventional method for the diagnosis of ehrlichia and anaplasma infections. This technique shows high sensitivity for the diagnosis of HME or HGA by demonstrating seroconversion or a 4-fold increase in titers between acute and convalescent phase sera. Serology for these infections is most commonly performed by indirect immunofluorescence antibody, or IFA. IFA is often considered the gold-standard serologic method, but it is labor intensive and requires highly trained personnel to perform testing and interpret the results. In addition, it is subjective and therefore prone to inter-reader and inter-laboratory variability.

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Conventional Methods for Diagnosis

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Jump to section:

• Introduction
• Outline
• Patient Case 1
• Laboratory Workup
• Conventional Methods for Diagnosis of Ehrlichiosis and Anaplasmosis
• Conventional Methods for Diagnosis of Ehrlichiosis and Anaplasmosis
• Conventional Methods for Diagnosis of Ehrlichiosis and Anaplasmosis
• Typical Positive Result by IFA
• Conventional Methods for Diagnosis of Ehrlichiosis and Anaplasmosis
• Diagnostic Tests for HME and HGA
• Conventional Methods for Diagnosis of Ehrlichiosis and Anaplasmosis
• Conventional Methods for Diagnosis of Ehrlichiosis and Anaplasmosis
• Giemsa-stained Preparation Slide
• Giemsa-stained Preparation Slide
• Patient Case 2
• Conventional Methods for Diagnosis of Babesia
• Conventional Methods for Diagnosis of Babesia
• Conventional Methods for Diagnosis of Babesia
• Conventional Methods for Diagnosis of Babesia
• Conventional Methods for Diagnosis of Babesia
• Patient Case 3
• Lyme Disease - Diagnostic Approach
• Conventional Methods for Diagnosis of Lyme Disease
• Patient Case 3 (continued)
• Diagnosis of Lyme Disease
• Lyme Disease - Serologic Assays
• Lyme Disease - Serologic Assays
• Lyme Disease - Serologic Assays
• Patient Case 3 (continued)
• Lyme Disease - Serologic Assays
• Patient Case 3 (continued)
• Lyme Disease - Factors to Consider
• Conclusions
• References
• Questions?