Laboratory Diagnosis of Tick-Borne Infections

Lyme Disease - Serologic Assays

July 2010

Regarding the screen enzyme immunoassay, the sensitivity of the assay may vary with the disease stage. As we can see in this table, the sensitivity of the screening EIA in stage 1 of the disease may be as low as 70-75%. In contrast, when testing is performed during stages 2 and 3 of Lyme disease, the sensitivity increases to greater than 90%. An additional point to consider is that early administration of antibiotics may weaken or delay the antibody response, and the sensitivity of EIA may be decreased. As we’ve already reviewed, specimens testing positive or equivocal by the screening method should be tested by Western blot.

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Lyme Disease - Serologic Assays

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Jump to section:

• Introduction
• Outline
• Patient Case 1
• Laboratory Workup
• Conventional Methods for Diagnosis of Ehrlichiosis and Anaplasmosis
• Conventional Methods for Diagnosis of Ehrlichiosis and Anaplasmosis
• Conventional Methods for Diagnosis of Ehrlichiosis and Anaplasmosis
• Typical Positive Result by IFA
• Conventional Methods for Diagnosis of Ehrlichiosis and Anaplasmosis
• Diagnostic Tests for HME and HGA
• Conventional Methods for Diagnosis of Ehrlichiosis and Anaplasmosis
• Conventional Methods for Diagnosis of Ehrlichiosis and Anaplasmosis
• Patient Case 2
• Giemsa-stained Preparation Slide
• Giemsa-stained Preparation Slide
• Conventional Methods for Diagnosis of Babesia
• Conventional Methods for Diagnosis of Babesia
• Conventional Methods for Diagnosis of Babesia
• Conventional Methods for Diagnosis of Babesia
• Conventional Methods for Diagnosis of Babesia
• Patient Case 3
• Lyme Disease - Diagnostic Approach
• Conventional Methods for Diagnosis of Lyme Disease
• Patient Case 3 (continued)
• Diagnosis of Lyme Disease
• Lyme Disease - Serologic Assays
• Lyme Disease - Serologic Assays
• Lyme Disease - Serologic Assays
• Patient Case 3 (continued)
• Lyme Disease - Serologic Assays
• Patient Case 3 (continued)
• Lyme Disease - Factors to Consider
• Conclusions
• References
• Questions?