Chronic Granulomatous Disease (CGD)

Neutrophil Oxidative Burst: Normal Individual

January 2010

Here is another example of the flow cytometric pattern associated with DHR oxidation by neutrophils to measure oxidative burst. In the laboratory test done at Mayo, the neutrophils are identified based on their size and granularity, using forward and side scatter measurements on the flow cytometer. The data in the 3 upper panels shows the identification of neutrophils in a whole blood sample along with the absence of any fluorescence in the unstimulated neutrophil population, which is in stark contrast to the lower panels, where the neutrophils on stimulation with PMA mount a strong oxidative burst with a complete right shift in fluorescence. 

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Neutrophil Oxidative Burst

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• Introduction
• Primary Immunodeficiencies (PIDs): What Are They?
• Primary Immunodeficiencies (PIDs): What Are They?
• Relative Distribution of the PIDs
• Components and Kinetics of the Immune Response
• Mechanisms of Innate Immunity
• Defect in the Innate Immune System: Chronic Granulomatous Disease
• Molecular Pathogenesis
• Clinical Features
• Laboratory Diagnosis of Neutrophil Oxidative Burst
• Nitro Blue-Tetrazolium Test (NBT)
• Dihydrorhodamine (DHR) Flow Cytometric Assay for Diagnosis of CGD
• Neutrophil Oxidative Burst: Normal Individual
• X-linked CGD
• Symptomatic Female Carrier with CGD
• Autosomal Recessive CGD
• Autosomal Recessive CGD
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