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Chronic Granulomatous Disease (CGD)

Clinical Features and Laboratory Testing



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Neutrophil Oxidative Burst: Normal Individual

Slide 13

January 2010

Here is another example of the flow cytometric pattern associated with DHR oxidation by neutrophils to measure oxidative burst. In the laboratory test done at Mayo, the neutrophils are identified based on their size and granularity, using forward and side scatter measurements on the flow cytometer. The data in the 3 upper panels shows the identification of neutrophils in a whole blood sample along with the absence of any fluorescence in the unstimulated neutrophil population, which is in stark contrast to the lower panels, where the neutrophils on stimulation with PMA mount a strong oxidative burst with a complete right shift in fluorescence. 

Neutrophil Oxidative Burst

 


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