Chronic Granulomatous Disease (CGD)
Clinical Features and Laboratory Testing
Nitro Blue-Tetrazolium Test (NBT)
January 2010
The NBT test is a semiquantitative method for evaluating neutrophil oxidative burst dysfunction in CGD patients.
The panel on the right demonstrates that neutrophils ingest the dye, nitroblue tetrazolium, and in the presence of reactive oxygen species, the yellow colored NBT compound is converted to the purple-blue formazan compound.
The panel on the left demonstrates that neutrophils from CGD patients fail to generate reactive oxygen species and therefore, cannot reduce the yellow NBT dye and thus there is no change in the color produced.
NBT |
Jump to section:
- Introduction
- Primary Immunodeficiencies (PIDs): What Are They?
- Primary Immunodeficiencies (PIDs): What Are They?
- Relative Distribution of the PIDs
- Components and Kinetics of the Immune Response
- Mechanisms of Innate Immunity
- Defect in the Innate Immune System: Chronic Granulomatous Disease
- Molecular Pathogenesis
- Clinical Features
- Laboratory Diagnosis of Neutrophil Oxidative Burst
- Nitro Blue-Tetrazolium Test (NBT)
- Dihydrorhodamine (DHR) Flow Cytometric Assay for Diagnosis of CGD
- Neutrophil Oxidative Burst: Normal Individual
- X-linked CGD
- Symptomatic Female Carrier with CGD
- Autosomal Recessive CGD
- Autosomal Recessive CGD
- Laboratory Test Ordering Information
- Questions?
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