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Chronic Granulomatous Disease (CGD)

Clinical Features and Laboratory Testing


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Nitro Blue-Tetrazolium Test (NBT)

Slide 11

January 2010

The NBT test is a semiquantitative method for evaluating neutrophil oxidative burst dysfunction in CGD patients.

The panel on the right demonstrates that neutrophils ingest the dye, nitroblue tetrazolium, and in the presence of reactive oxygen species, the yellow colored NBT compound is converted to the purple-blue formazan compound.

The panel on the left demonstrates that neutrophils from CGD patients fail to generate reactive oxygen species and therefore, cannot reduce the yellow NBT dye and thus there is no change in the color produced.  



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