Chronic Granulomatous Disease (CGD)
Clinical Features and Laboratory Testing
Laboratory Diagnosis of Neutrophil Oxidative Burst
January 2010
There are a few different methods available for the laboratory testing of neutrophil function as a diagnostic test for CGD.
The primary endpoint of lab testing in CGD is the assessment of the neutrophil oxidative burst since the molecular defects are confined to this enzyme complex.
The 3 major tests that have been used for clinical diagnostic purposes in CGD include the:
- Reduction of Nitro Blue-Tetrazolium (NBT) – performed on slides
- Measurement of superoxide production by visible spectrophotometry
- Measurement of Reactive oxygen species (ROS) production by flow cytometry, fluorometry or chemiluminescence
Laboratory Diagnosis |
Jump to section:
- Introduction
- Primary Immunodeficiencies (PIDs): What Are They?
- Primary Immunodeficiencies (PIDs): What Are They?
- Relative Distribution of the PIDs
- Components and Kinetics of the Immune Response
- Mechanisms of Innate Immunity
- Defect in the Innate Immune System: Chronic Granulomatous Disease
- Molecular Pathogenesis
- Clinical Features
- Laboratory Diagnosis of Neutrophil Oxidative Burst
- Nitro Blue-Tetrazolium Test (NBT)
- Dihydrorhodamine (DHR) Flow Cytometric Assay for Diagnosis of CGD
- Neutrophil Oxidative Burst: Normal Individual
- X-linked CGD
- Symptomatic Female Carrier with CGD
- Autosomal Recessive CGD
- Autosomal Recessive CGD
- Laboratory Test Ordering Information
- Questions?
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