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Chronic Granulomatous Disease (CGD)

Clinical Features and Laboratory Testing



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Laboratory Diagnosis of Neutrophil Oxidative Burst

Slide 10

January 2010

There are a few different methods available for the laboratory testing of neutrophil function as a diagnostic test for CGD.

The primary endpoint of lab testing in CGD is the assessment of the neutrophil oxidative burst since the molecular defects are confined to this enzyme complex.

The 3 major tests that have been used for clinical diagnostic purposes in CGD include the:

  • Reduction of Nitro Blue-Tetrazolium (NBT) – performed on slides
  • Measurement of superoxide production by visible spectrophotometry
  • Measurement of Reactive oxygen species (ROS) production by flow cytometry, fluorometry or chemiluminescence

Laboratory Diagnosis

 


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