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Red Cell Folate Testing

Unwarranted and Overutilized in the Era of Folic Acid Supplementation


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Quantitation of Folate: Methods

Slide 8

November 2010

There are a number of methods which can be utilized to quantitate serum or red cell folate. These include microbiologic assays, competitive protein-binding assays, or chromatography. A majority of laboratories, including Mayo, use a protein-binding assay with chemiluminescent detection for both serum and red blood cell folate, where the red cell hemolysate is prepared by lysing with ascorbic acid, which releases the intracellular folate, again primarily in the 5-tetrahydrofolate form.

Quantitation of Folate: Methods


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