QuantiFERON-TB Gold In-Tube

QuantiFERON-TB Gold In-Tube, cont.

November 2009

Unlike the first two generations of QuantiFERON, In-tube utilizes a specialized, 3 tube collection set. The first tube has a gray top, and is called the Nil tube. The Nil tube serves as the negative control, and no interferon gamma production, or very limited interferon gamma production, should be observed with the Nil tube. The second tube has a red top and is the TB-antigen tube. The antigen tube is coated with the lyophilized TB antigens ESAT-6, CFP-10 and TB 7.7. These antigens, when mixed with blood containing sensitized T cells, will lead to the production and secretion of interferon gamma. And finally, the third tube is called the Mitogen tube, and it has a purple top. The Mitogen tube contains phytohemagglutinin, which acts as a positive control for the production of interferon gamma from T cells.

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QuantiFERON-TB Gold In-Tube, cont.

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Jump to section:

• Introduction
• The Global Impact of Tuberculosis
• Conventional Diagnosis of Latent Tuberculosis Infection (LTBI)
• Limitations of TST
• Interferon Gamma Release Assays (IGRA): An Alternative to TST?
• QuantiFERON-TB Gold In-Tube
• QuantiFERON-TB Gold In-Tube, cont.
• QuantiFERON-TB Gold In-Tube, cont.
• QuantiFERON-TB Gold In-Tube Advantages over TST
• QuantiFERON-TB Gold In-Tube Limitations
• QuantiFERON-TB Gold In-Tube Applications
• QuantiFERON-TB Gold In-Tube Applications, cont.
• QuantiFERON-TB Gold In-Tube Applications, cont.
• QuantiFERON-TB Gold In-Tube Applications, cont.
• QuantiFERON-TB Gold In-Tube Applications, cont.
• QuantiFERON-TB Gold In-Tube Applications, cont.
• QuantiFERON-TB Gold In-Tube Applications, cont.
• QuantiFERON-TB Gold In-Tube Applications, cont.
• Information for Collection Sites Keys to Accurate Performance
• Blood Collection
• Blood Collection, cont.
• Blood Collection, cont.
• Blood Collection, cont.
• Mixing of Blood Collection Set
• Mixing of Blood Collection Set
• Storage and Incubation
• Storage and Incubation
• Incubation
• Centrifugation of Collection Tubes
• Shipping Specimens
• QuantiFERON-TB Gold In-Tube Conclusions
• Questions?