QuantiFERON-TB Gold In-Tube

QuantiFERON-TB Gold In-Tube

November 2009

Now that we have a general understanding for the principle behind this technology, I’d like to turn our focus to a particular interferon gamma release assay, called QuantiFERON TB Gold In-Tube, which we’ve recently implemented into our laboratory at Mayo Clinic. QuantiFERON is an in vitro whole blood test that is designed for use as an aid in diagnosing both latent and active TB disease.

There have been several versions of QuantiFERON since its initial release in 2001, but the latest version, known as QuantiFERON In-Tube, received FDA clearance in October of 2007. This 3rd generation assay offers several advantages over its predecessors, including an extended transport time from the collection site to the testing laboratory. QuantiFERON In-tube uses three peptide simulating antigens, including ESAT-6, CFP-10, and TB7.7, for the stimulation of T cells and subsequent secretion of interferon gamma.

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Jump to section:

• Introduction
• The Global Impact of Tuberculosis
• Conventional Diagnosis of Latent Tuberculosis Infection (LTBI)
• Limitations of TST
• Interferon Gamma Release Assays (IGRA): An Alternative to TST?
• QuantiFERON-TB Gold In-Tube
• QuantiFERON-TB Gold In-Tube, cont.
• QuantiFERON-TB Gold In-Tube, cont.
• QuantiFERON-TB Gold In-Tube Advantages over TST
• QuantiFERON-TB Gold In-Tube Limitations
• QuantiFERON-TB Gold In-Tube Applications
• QuantiFERON-TB Gold In-Tube Applications, cont.
• QuantiFERON-TB Gold In-Tube Applications, cont.
• QuantiFERON-TB Gold In-Tube Applications, cont.
• QuantiFERON-TB Gold In-Tube Applications, cont.
• QuantiFERON-TB Gold In-Tube Applications, cont.
• QuantiFERON-TB Gold In-Tube Applications, cont.
• QuantiFERON-TB Gold In-Tube Applications, cont.
• Information for Collection Sites Keys to Accurate Performance
• Blood Collection
• Blood Collection, cont.
• Blood Collection, cont.
• Blood Collection, cont.
• Mixing of Blood Collection Set
• Mixing of Blood Collection Set
• Storage and Incubation
• Storage and Incubation
• Incubation
• Centrifugation of Collection Tubes
• Shipping Specimens
• QuantiFERON-TB Gold In-Tube Conclusions
• Questions?