DNA Testing - The Next Generation

Greatest Impact of the Human Genome Project

May 2009

The very first impact was that high throughput methodologies came into place and these technologies were rapidly developed. Well, in 2001 the cost of the human genome was about $200 million dollars. But how much does that cost today - because it always costs the most to do something the first time. This is where the technology of NextGen Sequencers comes in. One of the key things of the ABI 3700 was the fact that multiple samples were processed simultaneously. Instead of a single sample at a time, 96 samples were done together.

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Jump to section:

• Introduction
• The Human Genome Project
• ABI 3700 Machine
• Celera Genomics
• Greatest Impact of the Human Genome Project
• Technological Advances
• Process Overview
• One Fragment = One Bead = One Read
• Complete Workflow
• Capability of This Machine
• What's Been Accomplished with the 454?
• Illumina Genome Analyzer
• Genome Analyzer Workflow
• Flow Cell Preparation
• Generate Samples
• Raw Data is Images
• Sequence Construction
• What's Been Accomplished with the Illumina Machine?
• SOLiD System - Overview
• SOLiD System 2.0 - Launch May 2008
• SOLiD System Summary Overview
• Higher Accuracy - New Probe Mix 1,2 Probes (Version 2)
• Higher Accuracy - New Probe Mix 1,2 Probes (Version 2)
• Sequential Rounds of Sequencing 1,2 Probes (Version 2)
• So, What Can You Do with a NextGen Sequencer?
• Whole Genomes
• Detecting Rare Mutations
• Mate-pair Reads
• Hybrid Selection
• Bar Coding
• Clinical Diagnostics
• Next-NextGen?
• Center for Individualized Medicine (CIM)
• Generating a Data Source
• Capabilities Grow Exponentially
• So, What's Coming?
• Building the Infrastructure for NextGen (and Beyond) Sequencing
• Acknowledgments
• Questions?