DNA Testing - The Next Generation
High Throughput, High Content Technologies
Celera Genomics
May 2009
That was done and developed by Craig Venter. His genome was the genome which was sequenced. They utilized whole genome shotgun sequencing with 330 ABI 3700’s, small insert libraries were then cloned, robotics were used to pick the colonies, and then after all the sequence was generated, a supercomputer was used for the assembly. Hence, in three months of sequencing, the 20+ GBs of Craig Venter’s genome was sequenced and this generated the first referenced human genome sequence. This was very impressive technology but technology moves on.
Celera Genomics |
Jump to section:
- Introduction
- The Human Genome Project
- ABI 3700 Machine
- Celera Genomics
- Greatest Impact of the Human Genome Project
- Technological Advances
- Process Overview
- One Fragment = One Bead = One Read
- Complete Workflow
- Capability of This Machine
- What's Been Accomplished with the 454?
- Illumina Genome Analyzer
- Genome Analyzer Workflow
- Flow Cell Preparation
- Generate Samples
- Raw Data is Images
- Sequence Construction
- What's Been Accomplished with the Illumina Machine?
- SOLiD System - Overview
- SOLiD System 2.0 - Launch May 2008
- SOLiD System Summary Overview
- Higher Accuracy - New Probe Mix 1,2 Probes (Version 2)
- Higher Accuracy - New Probe Mix 1,2 Probes (Version 2)
- Sequential Rounds of Sequencing 1,2 Probes (Version 2)
- So, What Can You Do with a NextGen Sequencer?
- Whole Genomes
- Detecting Rare Mutations
- Mate-pair Reads
- Hybrid Selection
- Bar Coding
- Clinical Diagnostics
- Next-NextGen?
- Center for Individualized Medicine (CIM)
- Generating a Data Source
- Capabilities Grow Exponentially
- So, What's Coming?
- Building the Infrastructure for NextGen (and Beyond) Sequencing
- Acknowledgments
- Questions?
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