DNA Testing - The Next Generation

ABI 3700 Machine

May 2009

And the state-of-the-art machine that enabled the sequencing of the human genome was the ABI 3700 which was a machine which 10 years ago cost $250 million. The capability of this machine was that 96 samples could be run simultaneously through very long capillaries and a 3-hour run time enabled, with hundreds of these machines running simultaneously, the complete sequencing of the first reference genome in three months time.

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Jump to section:

• Introduction
• The Human Genome Project
• ABI 3700 Machine
• Celera Genomics
• Greatest Impact of the Human Genome Project
• Technological Advances
• Process Overview
• One Fragment = One Bead = One Read
• Complete Workflow
• Capability of This Machine
• What's Been Accomplished with the 454?
• Illumina Genome Analyzer
• Genome Analyzer Workflow
• Flow Cell Preparation
• Generate Samples
• Raw Data is Images
• Sequence Construction
• What's Been Accomplished with the Illumina Machine?
• SOLiD System - Overview
• SOLiD System 2.0 - Launch May 2008
• SOLiD System Summary Overview
• Higher Accuracy - New Probe Mix 1,2 Probes (Version 2)
• Higher Accuracy - New Probe Mix 1,2 Probes (Version 2)
• Sequential Rounds of Sequencing 1,2 Probes (Version 2)
• So, What Can You Do with a NextGen Sequencer?
• Whole Genomes
• Detecting Rare Mutations
• Mate-pair Reads
• Hybrid Selection
• Bar Coding
• Clinical Diagnostics
• Next-NextGen?
• Center for Individualized Medicine (CIM)
• Generating a Data Source
• Capabilities Grow Exponentially
• So, What's Coming?
• Building the Infrastructure for NextGen (and Beyond) Sequencing
• Acknowledgments
• Questions?