DNA Testing - The Next Generation

Raw Data is Images

May 2009

Then the individual samples can be done with four different fluorescently colored nucleotides and if a nucleotide is added to one of these, that tells you the first base which is being added. You can see four different bases being added to these four different forests. You simply take a picture of this after each base addition. The bases are blocked so no additional base can be added. After you’ve take the picture you deblock the bases and you add the next base.

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Jump to section:

• Introduction
• The Human Genome Project
• ABI 3700 Machine
• Celera Genomics
• Greatest Impact of the Human Genome Project
• Technological Advances
• Process Overview
• One Fragment = One Bead = One Read
• Complete Workflow
• Capability of This Machine
• What's Been Accomplished with the 454?
• Illumina Genome Analyzer
• Genome Analyzer Workflow
• Flow Cell Preparation
• Generate Samples
• Raw Data is Images
• Sequence Construction
• What's Been Accomplished with the Illumina Machine?
• SOLiD System - Overview
• SOLiD System 2.0 - Launch May 2008
• SOLiD System Summary Overview
• Higher Accuracy - New Probe Mix 1,2 Probes (Version 2)
• Higher Accuracy - New Probe Mix 1,2 Probes (Version 2)
• Sequential Rounds of Sequencing 1,2 Probes (Version 2)
• So, What Can You Do with a NextGen Sequencer?
• Whole Genomes
• Detecting Rare Mutations
• Mate-pair Reads
• Hybrid Selection
• Bar Coding
• Clinical Diagnostics
• Next-NextGen?
• Center for Individualized Medicine (CIM)
• Generating a Data Source
• Capabilities Grow Exponentially
• So, What's Coming?
• Building the Infrastructure for NextGen (and Beyond) Sequencing
• Acknowledgments
• Questions?