DNA Testing - The Next Generation

Flow Cell Preparation

May 2009

The way one generates these samples is that a single-stranded molecule of DNA has linkers adapted on both sides. One of those linkers is complementary to the same color linker which is down on a slide and that sample can then fold over to make a bridge. Now you have a template and a primer so that if you add the corresponding nucleotides, you can make a copy of that. When one denatures that, one has two strands where you started with one and you continue this process for a series of times so that ultimately you generate a forest. A forest is an amplification of an original DNA fragment so that one has some 10,000 copies of that fragment.

< Previous Slide

Flow Cell Preparation

Next Slide >

 

Jump to section:

• Introduction
• The Human Genome Project
• ABI 3700 Machine
• Celera Genomics
• Greatest Impact of the Human Genome Project
• Technological Advances
• Process Overview
• One Fragment = One Bead = One Read
• Complete Workflow
• Capability of This Machine
• What's Been Accomplished with the 454?
• Illumina Genome Analyzer
• Genome Analyzer Workflow
• Flow Cell Preparation
• Generate Samples
• Raw Data is Images
• Sequence Construction
• What's Been Accomplished with the Illumina Machine?
• SOLiD System - Overview
• SOLiD System 2.0 - Launch May 2008
• SOLiD System Summary Overview
• Higher Accuracy - New Probe Mix 1,2 Probes (Version 2)
• Higher Accuracy - New Probe Mix 1,2 Probes (Version 2)
• Sequential Rounds of Sequencing 1,2 Probes (Version 2)
• So, What Can You Do with a NextGen Sequencer?
• Whole Genomes
• Detecting Rare Mutations
• Mate-pair Reads
• Hybrid Selection
• Bar Coding
• Clinical Diagnostics
• Next-NextGen?
• Center for Individualized Medicine (CIM)
• Generating a Data Source
• Capabilities Grow Exponentially
• So, What's Coming?
• Building the Infrastructure for NextGen (and Beyond) Sequencing
• Acknowledgments
• Questions?