DNA Testing - The Next Generation

Capability of This Machine

May 2009

The capability of this machine when it first came out - it was called the Genome Sequencer 20 because it could do 20 million base pairs of sequencing. This was a machine that was 100 times more powerful than the ABI 3700, its predecessor. But very quickly this machine was upgraded; 5 times greater so that it could do 100 to 200 million base pairs of sequencing in just 12 hours and with this type of machine, 10 of these machines could do the human genome in just 10 days and it would only take 2 to 3 people to run this. The next upgrade, which has just become available, is called the GS FLX Titanium. This will do 500 Mbs of sequencing in 12 hours.

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Jump to section:

• Introduction
• The Human Genome Project
• ABI 3700 Machine
• Celera Genomics
• Greatest Impact of the Human Genome Project
• Technological Advances
• Process Overview
• One Fragment = One Bead = One Read
• Complete Workflow
• Capability of This Machine
• What's Been Accomplished with the 454?
• Illumina Genome Analyzer
• Genome Analyzer Workflow
• Flow Cell Preparation
• Generate Samples
• Raw Data is Images
• Sequence Construction
• What's Been Accomplished with the Illumina Machine?
• SOLiD System - Overview
• SOLiD System 2.0 - Launch May 2008
• SOLiD System Summary Overview
• Higher Accuracy - New Probe Mix 1,2 Probes (Version 2)
• Higher Accuracy - New Probe Mix 1,2 Probes (Version 2)
• Sequential Rounds of Sequencing 1,2 Probes (Version 2)
• So, What Can You Do with a NextGen Sequencer?
• Whole Genomes
• Detecting Rare Mutations
• Mate-pair Reads
• Hybrid Selection
• Bar Coding
• Clinical Diagnostics
• Next-NextGen?
• Center for Individualized Medicine (CIM)
• Generating a Data Source
• Capabilities Grow Exponentially
• So, What's Coming?
• Building the Infrastructure for NextGen (and Beyond) Sequencing
• Acknowledgments
• Questions?