DNA Testing - The Next Generation
High Throughput, High Content Technologies
Introduction
May 2009
Welcome to Mayo Medical Laboratories' Hot Topics. These presentations provide short discussion of current topics and may be helpful to you in your practice.
Our presenter for this program is Dr. David I. Smith, PhD, from the Division of Experimental Pathology and Laboratory Medicine at Mayo Clinic. Technology advances are rapidly expanding our ability to detect genomic differences in normal and disease states. Dr. Smith reviews how these technological improvements have increased the speed and capacity of DNA sequencers, expectations for future improvements, and the impact that this will have on clinical practice.
Introduction |
Jump to section:
- The Human Genome Project
- ABI 3700 Machine
- Celera Genomics
- Greatest Impact of the Human Genome Project
- Technological Advances
- Process Overview
- One Fragment = One Bead = One Read
- Complete Workflow
- Capability of This Machine
- What's Been Accomplished with the 454?
- Illumina Genome Analyzer
- Genome Analyzer Workflow
- Flow Cell Preparation
- Generate Samples
- Raw Data is Images
- Sequence Construction
- What's Been Accomplished with the Illumina Machine?
- SOLiD System - Overview
- SOLiD System 2.0 - Launch May 2008
- SOLiD System Summary Overview
- Higher Accuracy - New Probe Mix 1,2 Probes (Version 2)
- Higher Accuracy - New Probe Mix 1,2 Probes (Version 2)
- Sequential Rounds of Sequencing 1,2 Probes (Version 2)
- So, What Can You Do with a NextGen Sequencer?
- Whole Genomes
- Detecting Rare Mutations
- Mate-pair Reads
- Hybrid Selection
- Bar Coding
- Clinical Diagnostics
- Next-NextGen?
- Center for Individualized Medicine (CIM)
- Generating a Data Source
- Capabilities Grow Exponentially
- So, What's Coming?
- Building the Infrastructure for NextGen (and Beyond) Sequencing
- Acknowledgments
- Questions?
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