The Human Genome Project
Now, again, one of the problems is that here is a very good example of a single gene. The gene is shown with the exons being in blue but that one gene is not making one transcript. Actually, alternate splicing enables you to make multiple distinct transcripts from that same gene and in this instance this gene could be making 50 different proteins. If we were trying to measure what was going on with microarrays, it is very difficult to tell which of these isoforms are produced. If we go to the protein itself, and we discover that one of these proteins is an important marker for how a patient is going to do, we could possibly detect it.
Example of a Single Gene
Jump to section:
- How Do We Obtain Genetic Information?
- Cell Cross-Section
- Different Cell Types
- What Happens When You Sit Outside in the Sun?
- DNA is the Altered Target in Cancer Cells
- DNA Structure
- How to Tackle a Problem as Difficult as Cancer?
- Sequencing DNA
- More on DNA Structure
- Replicating a Strand of DNA
- Developing the Deoxy Chain Terminiation Sequence
- Reading a DNA Sequence
- Gel Electrophoresis
- Two DNA Sequences Seen in Gel Electrophoresis
- Overlapping Pieces of DNA
- Requirements to Sequence the Human Genome?
- Advances in Sanger Sequencing
- Sequencing with Fluorescent Dye
- Advances in Fluorescent Sequencing
- Celera Genomics
- Capacity: 96 Capillary Sequencing
- Computers and the Human Genome Project
- Where are We Today?
- What Have We Learned From Genome Sequences?
- What Can We Do With Sequenced Genomes?
- Transcriptional Profiling (TP)
- Different Technologies to Produce Microarrays
- Utilizing Microarrays to Measure Gene Expression
- Hyrbidization to an Affymetrix Array
- Gene Expression Comparison Between Samples
- Gene Expression Map
- Proteomic-Based Strategies
- Example of a Single Gene
- How Do We Quantify Proteins?
- Differentiate Between Control and Disease State
- Mass Spectrometry
- Electrospray Ionization FT-ICR Mass Spectrometer
- LC-ESI-TOF vs LC-FT-ICR Mass Spectrometry
- What's the Short-term Payoff?
- What's the Long-term Payoff?
- Diagram of Pathways Involved in Steroid Metabolism