The Human Genome Project
Capacity: 96 Capillary Sequencing
March 2009
The capacity of 96 capillary sequencing is that with a 3-hour run time, one can do 8 cycles a day. Each machine can run 960 sequences a day. Hence the 330 machines that Celera had were able to process 316,800 sequences with up to 1000 base pairs per sequence. Thus, that is 31,680,000 base pairs of sequencing per day and when this was run continuously for 3 to 4 months, they were able to generate the complete human genome sequence of the Craig Venter genome and basically do 25 gigabases of sequencing to completely cover his genome.
Capacity: 96 Capillary Sequencing |
Jump to section:
- Introduction
- How Do We Obtain Genetic Information?
- Cell Cross-Section
- Different Cell Types
- What Happens When You Sit Outside in the Sun?
- Melanoma
- DNA is the Altered Target in Cancer Cells
- DNA Structure
- How to Tackle a Problem as Difficult as Cancer?
- Sequencing DNA
- More on DNA Structure
- Replicating a Strand of DNA
- Developing the Deoxy Chain Terminiation Sequence
- Reading a DNA Sequence
- Gel Electrophoresis
- Two DNA Sequences Seen in Gel Electrophoresis
- Overlapping Pieces of DNA
- Requirements to Sequence the Human Genome?
- Advances in Sanger Sequencing
- Sequencing with Fluorescent Dye
- Advances in Fluorescent Sequencing
- Celera Genomics
- Capacity: 96 Capillary Sequencing
- Computers and the Human Genome Project
- Where are We Today?
- What Have We Learned From Genome Sequences?
- What Can We Do With Sequenced Genomes?
- Transcriptional Profiling (TP)
- Different Technologies to Produce Microarrays
- Utilizing Microarrays to Measure Gene Expression
- Hyrbidization to an Affymetrix Array
- Gene Expression Comparison Between Samples
- Gene Expression Map
- Proteomic-Based Strategies
- Example of a Single Gene
- Proteomics
- How Do We Quantify Proteins?
- Differentiate Between Control and Disease State
- Mass Spectrometry
- Electrospray Ionization FT-ICR Mass Spectrometer
- LC-ESI-TOF vs LC-FT-ICR Mass Spectrometry
- What's the Short-term Payoff?
- What's the Long-term Payoff?
- Diagram of Pathways Involved in Steroid Metabolism
- Questions?
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