The Human Genome Project
When this was first developed it was still done still with polyacrylamide gels. That would still require many gels; that would still require multiple millions of gels. But very quickly people switched that instead of gels they switched to long capillary tubes that the samples could be run through. These capillary tubes enable you to reuse them many times. You put a sample on, you run the sample through, you wash to remove anything residual, and you add an additional sample. Then you are not required to continually prepare new polyacrylamide gels and this sets you up for automation. Initially these were single capillary devices that would run one sample through, again one sample could do a complete sequence of one material but then very quickly they developed 6-capillary devices and eventually this led to a 96-capillary device.
Advances in Fluorescent Sequencing
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- How Do We Obtain Genetic Information?
- Cell Cross-Section
- Different Cell Types
- What Happens When You Sit Outside in the Sun?
- DNA is the Altered Target in Cancer Cells
- DNA Structure
- How to Tackle a Problem as Difficult as Cancer?
- Sequencing DNA
- More on DNA Structure
- Replicating a Strand of DNA
- Developing the Deoxy Chain Terminiation Sequence
- Reading a DNA Sequence
- Gel Electrophoresis
- Two DNA Sequences Seen in Gel Electrophoresis
- Overlapping Pieces of DNA
- Requirements to Sequence the Human Genome?
- Advances in Sanger Sequencing
- Sequencing with Fluorescent Dye
- Advances in Fluorescent Sequencing
- Celera Genomics
- Capacity: 96 Capillary Sequencing
- Computers and the Human Genome Project
- Where are We Today?
- What Have We Learned From Genome Sequences?
- What Can We Do With Sequenced Genomes?
- Transcriptional Profiling (TP)
- Different Technologies to Produce Microarrays
- Utilizing Microarrays to Measure Gene Expression
- Hyrbidization to an Affymetrix Array
- Gene Expression Comparison Between Samples
- Gene Expression Map
- Proteomic-Based Strategies
- Example of a Single Gene
- How Do We Quantify Proteins?
- Differentiate Between Control and Disease State
- Mass Spectrometry
- Electrospray Ionization FT-ICR Mass Spectrometer
- LC-ESI-TOF vs LC-FT-ICR Mass Spectrometry
- What's the Short-term Payoff?
- What's the Long-term Payoff?
- Diagram of Pathways Involved in Steroid Metabolism