The Human Genome Project
Sequencing with Fluorescent Dye
March 2009
This is a result from a sequencing with the fluorescent dyes. One of the things that you can see is that this is a single lane on this and you can still see all 4 of the nucleotides together. This reads some 300 bases of a sequence and this enables the ability for this to be developed into a high throughput technology.
Sequencing with Fluorescent Dye |
Jump to section:
- Introduction
- How Do We Obtain Genetic Information?
- Cell Cross-Section
- Different Cell Types
- What Happens When You Sit Outside in the Sun?
- Melanoma
- DNA is the Altered Target in Cancer Cells
- DNA Structure
- How to Tackle a Problem as Difficult as Cancer?
- Sequencing DNA
- More on DNA Structure
- Replicating a Strand of DNA
- Developing the Deoxy Chain Terminiation Sequence
- Reading a DNA Sequence
- Gel Electrophoresis
- Two DNA Sequences Seen in Gel Electrophoresis
- Overlapping Pieces of DNA
- Requirements to Sequence the Human Genome?
- Advances in Sanger Sequencing
- Sequencing with Fluorescent Dye
- Advances in Fluorescent Sequencing
- Celera Genomics
- Capacity: 96 Capillary Sequencing
- Computers and the Human Genome Project
- Where are We Today?
- What Have We Learned From Genome Sequences?
- What Can We Do With Sequenced Genomes?
- Transcriptional Profiling (TP)
- Different Technologies to Produce Microarrays
- Utilizing Microarrays to Measure Gene Expression
- Hyrbidization to an Affymetrix Array
- Gene Expression Comparison Between Samples
- Gene Expression Map
- Proteomic-Based Strategies
- Example of a Single Gene
- Proteomics
- How Do We Quantify Proteins?
- Differentiate Between Control and Disease State
- Mass Spectrometry
- Electrospray Ionization FT-ICR Mass Spectrometer
- LC-ESI-TOF vs LC-FT-ICR Mass Spectrometry
- What's the Short-term Payoff?
- What's the Long-term Payoff?
- Diagram of Pathways Involved in Steroid Metabolism
- Questions?
External
link
PDF
document
Excel
document
Word
document