The Human Genome Project
Gel Electrophoresis
March 2009
They are then added to a gel electrophoresis apparatus where you put a little bit of buffer on the top, a little bit on the bottom. You attach electrodes so that the positive electrode is towards the bottom. Since the DNA is negatively charged, it will be attracted to the positive electrode and the various DNA fragments that have synthesized will be migrating through the gel. And the only thing is that the fragments are able to traverse the gel more quickly than the larger fragments so that one can see the single base at the very bottom of the gel 2, 3, 4, etc. all the way up the gel and then one simply looks at where one is along the gel and one can determine the length of the growing chain. When this is done, you simply open the gel up.
Gel Electrophoresis |
Jump to section:
- Introduction
- How Do We Obtain Genetic Information?
- Cell Cross-Section
- Different Cell Types
- What Happens When You Sit Outside in the Sun?
- Melanoma
- DNA is the Altered Target in Cancer Cells
- DNA Structure
- How to Tackle a Problem as Difficult as Cancer?
- Sequencing DNA
- More on DNA Structure
- Replicating a Strand of DNA
- Developing the Deoxy Chain Terminiation Sequence
- Reading a DNA Sequence
- Gel Electrophoresis
- Two DNA Sequences Seen in Gel Electrophoresis
- Overlapping Pieces of DNA
- Requirements to Sequence the Human Genome?
- Advances in Sanger Sequencing
- Sequencing with Fluorescent Dye
- Advances in Fluorescent Sequencing
- Celera Genomics
- Capacity: 96 Capillary Sequencing
- Computers and the Human Genome Project
- Where are We Today?
- What Have We Learned From Genome Sequences?
- What Can We Do With Sequenced Genomes?
- Transcriptional Profiling (TP)
- Different Technologies to Produce Microarrays
- Utilizing Microarrays to Measure Gene Expression
- Hyrbidization to an Affymetrix Array
- Gene Expression Comparison Between Samples
- Gene Expression Map
- Proteomic-Based Strategies
- Example of a Single Gene
- Proteomics
- How Do We Quantify Proteins?
- Differentiate Between Control and Disease State
- Mass Spectrometry
- Electrospray Ionization FT-ICR Mass Spectrometer
- LC-ESI-TOF vs LC-FT-ICR Mass Spectrometry
- What's the Short-term Payoff?
- What's the Long-term Payoff?
- Diagram of Pathways Involved in Steroid Metabolism
- Questions?
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