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Vitamin D Testing

Analytical & Clinical Conundrums


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Lab Values After Vit-D Supplementation

Slide 21

January 2009

This graph shows the increase in vitamin D levels in this premature infant after supplementation. The green line describes an increase in vitamin D levels which increase up to 150 ng/mL at which the clinicians decided to stop the vitamin D supplementation. The blue line describes the vitamin D2 levels, which were determined using the LC-MS/MS method. The pink line describes vitamin D3 levels. The red dot, which is 1,25-dihydroxy vitamin D, was elevated when the vitamin D level and the calcium was low in this premature infant.

When the vitamin D levels reached normal levels, 1,25-dihydroxy vitamin D normalized. PTH and BAP levels were also normalized in this infant.

Lab Values After Supplementation


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