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Vitamin D Testing

Analytical & Clinical Conundrums


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Reference Ranges for 25-OH Vitamin D

Slide 14

January 2009

This slide shows the reference ranges for 25-hydroxy vitamin D. Less than 10 ng/mL describes severe deficiency, which could be associated with osteomalacia or rickets. 10-25 ng/mL will correspond to mild to moderate 25-hydroxy vitamin D deficiency, which may be associated with an increased risk of osteoporosis or secondary hyperparathyroidism. In our review of the literature, 25-80 ng/mL was considered optimum 25-hydroxy vitamin D levels. 80 ng/mL levels or above are considered toxic. 80 ng/mL is the lowest reported level associated with toxicity in patients without primary hyperparathyroidism and with normal renal function.

Reference Ranges


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