Vitamin D Testing

History of 25-OH Vitamin D Testing

January 2009

The endocrine lab at Mayo Clinic has a history of vitamin D testing since 1981. We were performing 25-hydroxy vitamin D testing by HPLC. Our volume in 1981 was 50 samples/day. IN 2000, we switched to RIA in 2000, and we were able to perform 150 samples/day. In 2004, we implemented LC-MS/MS into the endocrine lab and we were able to perform 400 samples/day. With this technology today, we are able to perform 3,000-3,500 samples a day to determine the level of 25-hydroxy vitamin D in the blood.

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History of Vitamin D Testing

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• Introduction
• Objectives
• Rickets
• UVB Light
• Vitamin D Deficiency
• Interaction Between Vitamin D and Calcium
• Vitamin D3
• Vitamin D2
• Various Vitamin D Tests
• History of 25-OH Vitamin D Testing
• Accreditation Agencies for Precision Among Peers
• Quality of the 25-OH Vitamin D Testing
• 25-OH Vitamin D Volume
• Reference Ranges for 25-OH Vitamin D
• Various Vitamin D Tests
• Case Study - Rickets in a Premature Infant
• Birth History
• Ionized Calcium
• Fractures Identified on Routine Radiographic Surveillance for Line Placement
• Vitamin D Supplementation
• Lab Values After Vit-D Supplementation
• Mother's History
• Infant's X-ray
• Mother's Creatinine
• The Connection Between the Mother and the Infant
• Summary
• Questions?