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Vitamin D Testing

Analytical & Clinical Conundrums


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History of 25-OH Vitamin D Testing

Slide 10

January 2009

The endocrine lab at Mayo Clinic has a history of vitamin D testing since 1981. We were performing 25-hydroxy vitamin D testing by HPLC. Our volume in 1981 was 50 samples/day. IN 2000, we switched to RIA in 2000, and we were able to perform 150 samples/day. In 2004, we implemented LC-MS/MS into the endocrine lab and we were able to perform 400 samples/day. With this technology today, we are able to perform 3,000-3,500 samples a day to determine the level of 25-hydroxy vitamin D in the blood.

History of Vitamin D Testing


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