Herpes Simplex and Varicella Zoster Viruses
Fast, Efficient, and Accurate Processing for PCR Detection
Introduction
December 2009
Welcome to Mayo Medical Laboratories' Hot Topics. These presentations provide short discussion of current topics and may be helpful to you in your practice.
Our presenter for this program is Dr. Bobbi Pritt, Assistant Professor of Laboratory Medicine and Pathology and Director of the Clinical Virology and Parasitology Laboratories in the Division of Clinical Microbiology at Mayo Clinic. Dr. Pritt will provide an overview of testing for Varicella-Zoster and Herpes Simplex Virus by Rapid PCR and explain the recent changes to specimen requirements for these assays.
Introduction |
Jump to section:
- The Viruses
- Laboratory Diagnosis
- HSV/VZV Real-time PCR Sample Workflow
- Is Extraction Required for Dermal and Anogenital Specimens?
- Direct Processing in Neutralization Buffer: An Alternative to MagNA Pure Extraction
- Match Up Identifying Information
- Remove Swab from the Culturette Tube
- Heat Swab in Neutralization Buffer
- Sample is Ready for PCR
- A New Workflow for Dermal and Anogenital Swabs
- Direct Processing in Neutralization Buffer: Comparable to DNA Extraction?
- Comparison of Detection - HSV
- Results: Comparison of Amplification Cycle Threshold
- Comparison of Detection - VZV
- Results: Comparison of Amplification Crossing Points
- Questions and Answers
- Questions and Answers
- M5 Media vs Culturette Tubes: Experiment
- M5 Media vs Culturette Tubes: Results
- Conclusions
- Questions?
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