Herpes Simplex and Varicella Zoster Viruses

Remove Swab from the Culturette Tube

December 2009

Next, the swab is removed from the culturette tube and placed into the tube with neutralization buffer. The shaft of the swab is clipped off as shown here, and the lid is then placed on the new tube. The clippers used to cut the swab shaft are cleaned thoroughly in bleach and ethanol between each patient sample, to prevent cross contamination between samples.

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Jump to section:

• Introduction
• The Viruses
• Laboratory Diagnosis
• HSV/VZV Real-time PCR Sample Workflow
• Is Extraction Required for Dermal and Anogenital Specimens?
• Direct Processing in Neutralization Buffer: An Alternative to MagNA Pure Extraction
• Match Up Identifying Information
• Remove Swab from the Culturette Tube
• Heat Swab in Neutralization Buffer
• Sample is Ready for PCR
• A New Workflow for Dermal and Anogenital Swabs
• Direct Processing in Neutralization Buffer: Comparable to DNA Extraction?
• Comparison of Detection - HSV
• Results: Comparison of Amplification Cycle Threshold
• Comparison of Detection - VZV
• Results: Comparison of Amplification Crossing Points
• Questions and Answers
• Questions and Answers
• M5 Media vs Culturette Tubes: Experiment
• M5 Media vs Culturette Tubes: Results
• Conclusions
• Questions?